RNA-Seq analysis of isolate- and growth phase-specific differences in the global transcriptomes of enteropathogenic Escherichia coli prototype isolates

نویسندگان

  • Tracy H. Hazen
  • Sean C. Daugherty
  • Amol Shetty
  • Anup A. Mahurkar
  • Owen White
  • James B. Kaper
  • David A. Rasko
چکیده

Enteropathogenic Escherichia coli (EPEC) are a leading cause of diarrheal illness among infants in developing countries. E. coli isolates classified as typical EPEC are identified by the presence of the locus of enterocyte effacement (LEE) and the bundle-forming pilus (BFP), and absence of the Shiga-toxin genes, while the atypical EPEC also encode LEE but do not encode BFP or Shiga-toxin. Comparative genomic analyses have demonstrated that EPEC isolates belong to diverse evolutionary lineages and possess lineage- and isolate-specific genomic content. To investigate whether this genomic diversity results in significant differences in global gene expression, we used an RNA sequencing (RNA-Seq) approach to characterize the global transcriptomes of the prototype typical EPEC isolates E2348/69, B171, C581-05, and the prototype atypical EPEC isolate E110019. The global transcriptomes were characterized during laboratory growth in two different media and three different growth phases, as well as during adherence of the EPEC isolates to human cells using in vitro tissue culture assays. Comparison of the global transcriptomes during these conditions was used to identify isolate- and growth phase-specific differences in EPEC gene expression. These analyses resulted in the identification of genes that encode proteins involved in survival and metabolism that were coordinately expressed with virulence factors. These findings demonstrate there are isolate- and growth phase-specific differences in the global transcriptomes of EPEC prototype isolates, and highlight the utility of comparative transcriptomics for identifying additional factors that are directly or indirectly involved in EPEC pathogenesis.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Transcriptional Variation of Diverse Enteropathogenic Escherichia coli Isolates under Virulence-Inducing Conditions

Enteropathogenic Escherichia coli (EPEC) bacteria are a diverse group of pathogens that cause moderate to severe diarrhea in young children in developing countries. EPEC isolates can be further subclassified as typical EPEC (tEPEC) isolates that contain the bundle-forming pilus (BFP) or as atypical EPEC (aEPEC) isolates that do not contain BFP. Comparative genomics studies have recently highlig...

متن کامل

Clonal Relatedness of Enterotoxigenic and Enteropathogenic Escherichia coli Isolates from Diverse Human, Foods and Calf Sources

Background: Foodborne infection caused by EnterotoxigenicEscherichia coli (ETEC) and Enteropathogenic Escherichia coli (EPEC) is one of the major health problems, particularly in the developing countries. Therefore, it is vital to identify the origin of food contamination to plan control strategies efficiently. Method: A tota...

متن کامل

بررسی فراوانی اشریشیاکلی انتروپاتوژنیک از نمونه های اسهال کودکان زیر 5 سال ناشی از طغیان های غذایی کشوری با روش مولکولی PCR

Background and Aim: Enteropathogenic Escherichia coli (EPEC) pathotypes belong to Enterobacteriaceae family that is known as the cause of gastroenteritis and diarrhea in under-5-year-old children. These bacteria have high prevalence in developed and developing countries that may cause severe illness or even death. The aim of this study was to examine EPEC prevalence in diarrheal samples of chil...

متن کامل

Pathovar Transcriptomes

Archetypal pathogenic bacterial strains are often used to elucidate regulatory networks of an entire pathovar, which encompasses multiple lineages and phylogroups. With enteropathogenic Escherichia coli (EPEC) as a model system, Hazen and colleagues (mSystems 6:e00024-17, 2017, https://doi.org/10.1128/mSystems.00024-17) used 9 isolates representing 8 lineages and 3 phylogroups to find that isol...

متن کامل

A STUDY OF SALMONELLA, SHIGELLA AND ENTEROPATHOGENIC ESCHERICHIA COLI SEROTYPES IN ACUTE GASTROENTERITIS CHILDREN UNDER THE AGE OF FIVE

In this study, it has been our purpose to isolate and serotype enteropathogenic E. coli, salmonella and shigella and determine their resistance to antibiotics. 502 swab specimens were collected during mid-July to midNovember. 1986. from two children's hospitals in Tenran. From 502 cultured specimens. 108 specimens were positive for salmonella. shigella and enteropathogenic E. coli. corresp...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2015